LABORATORY SAFETY MANUAL

SECTION I 

1. Introduction
2. Scope
3. Responsibilities
4. Raising Safety Concerns

SECTION II

1. Chemical Hygiene and Safety Plan
        a.     General Standard Operating Procedures       
2. Laboratory Housekeeping
3. Training Requirements
4. Hazardous Chemicals
5. Warning Labels
6. Material Safety Data Sheets
7. Toxicology Overview

	a.	Chemical Toxicity
	b.	Dose Response Relationships
	c.	Route of Entry
	d.	Health Effects
	e.	Other Factors Affecting Toxicity

8. Prior Approval
9. Research Protocol Form
10. Working in Laboratories After Regular Hours
11. Unattended Experiment Policy
12. Laboratory Security Policy

SECTION III

1. Personal Protective Equipment and Clothing

	a.	General Policy
	b.	Eye and Face Protection
	c.	Skin Protection
	d.	Respirators
	e.	Personal Protective Equipment (PPE) Hazard Assessment
	f.	Selection Chart for Eye and Face Protection

2. Laboratory Fume Hoods

SECTION IV

1. Fire Emergencies
2. Chemical Spills
3. Personal Contamination        

	a.	General Information
	b.	Chemicals Spilled on the Skin
	c.	Chemicals in the Eyes

4. Accident Reporting

	a.	Reporting Hazards
	b.	Hazardous Substance Exposure
	c.	Employee Accidents/Hazardous Substance Exposures
	d.	Student Accidents/Hazardous Substance Exposures
	e.	Reporting of "Near Miss" Accidents

SECTION V

1. Purchases, Donations and Transfers of Hazardous Materials
2. Purchase and Disposal of Controlled Substances
3. Chemical Storage and Transportation

	a.	General Considerations for Chemical Storage
	b.	Flammable and Combustible Liquid Storage
	c.	Chemical Stability
	d.	Shock Sensitive Chemicals
	e.	Compounds that form Peroxides during Storage
	f.	Compressed Gas Storage
	g.	Transportation of Hazardous Chemicals
	h.	Shipment of Biological Materials

SECTION VI

1. Waste Disposal
       a.     Introduction
       b.     Responsibilities
       c.     Packaging and Labeling Procedures
       d.     Waste Reduction
       e.     Definitions
2. Disposal Procedures for Electrophoresis Buffer Solutions and Gels
3. EHRS's Chemical/Hazardous Waste Checklist for Compliance
4. EPA's (Environmental Protection Agency) Hazardous Waste List
5. Laboratory Cleanout

SECTION VII

1. Safe Handling of Flammables
2. Safe Handling of Reactive and Peroxide Forming Chemicals
3. Safe Handling of Corrosive Chemicals
4. Safe Handling of Compressed Gases
5. Safe Handling of Nanomaterials
6. Standard Operating Procedures for Reproductive Hazards, Carcinogens, or Acutely Toxic Chemicals
7. Standard Operating Procedures for Hydrofluoric Acid
8. Standard Operating Procedures for Pyrophoric Chemicals
9. Standard Operating Procedures for Piranha Solutions
10. Standard Operating Procedures for Gel Electrophoresis
11. Standard Operating Procedures for Sodium Azide
12. Standard Operating Procedures for Cytotoxic Agents
13. Standard Operating Procedure for Water Sensitive Chemicals
14. Standard Operating Procedure for Handling Animals Dosed with Toxic Chemicals
15. Diseases Communicable From Animals to Humans
16. Recommended Procedures for Preventing the Production of Aerosols
17. Recommended Procedures for the Proper Use of Biological Safety Cabinets
18. Recommended Procedures for Autoclaving Materials

APPENDIX

1.List of Some Carcinogens and Suspect Carcinogens
2. List of Some Reproductive Hazards
3. List of Some Acutely Toxic Chemicals
4. Examples of Incompatible Chemicals
5. Compounds That Form Peroxide During Storage

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