BIOLOGICAL SAFETY MANUAL

  1. Definition of Biohazards and Potentially Infectious Material
  2. Procedures for Biohazard Control
  3. Class II Biological Safety Cabinets
  4. Posting of Biological Hazard Signs
  5.

Emergency Response

    a. Biohazard Spills inside a Biological Safety Cabinet
    b. Biohazard Spills outside a Biological Safety Cabinet
    c. Large Spill of Biosafety Level 2 Material (>500ml)
    d. Personal Contamination
    e. Decontamination and Clean Up of Blood Spills
  6. Biological Risk Assessments
    a. Quick Guide to Risk Assessment for Biological Hazards in the Laboratory
  7. Laboratory Security Policy
  8. Biohazardous Waste
    a. Definitions
  9. Procedures for the Safe Disposal of Biohazardous Waste
  10. Agents required to be Autoclaved or Decontaminated Prior to Disposal
  11. Decontamination, Sterilization and Disinfection
    a. General
    b. Definitions
    c. Methods
  12. Activity Levels of Selected Liquid Germicides
  13. Shipment of Biological Materials
    a. Table 1: Category A Infectious Substances
    b. Table 2: Shipping Summary Table
    c. Packing Instructions 602 Summary
    d. Packing Instructions 650 Summary
    e. Packing Instructions 904 Summary
    f. Packing Instructions 913 Summary
    g. Exempt Packing Instructions
  14. Tissue Culture/Cell Lines
  15. Liquid Nitrogen and Frozen Biological Material
  16. Research Involving Recombinant and Synthetic Nucleic Acid Molecules
  17. Institutional Biosafety Committee Information
  18. Summary of Recommended Biosafety Levels for Infectious Agents and Infected Animals
    a. Table 1 - Summary of Recommended Biosafety Levels for Infectious Agents
    b. Table II - Summary of Recommended Biosafety Levels for activities in which experimentally or naturally infected vertebrate animals are used
  19. Laboratory Practices for each Biosafety Level and Biosafety Level Criteria
  20. Vertebrate Animal Biosafety Level Criteria for Vivarium Research Facilities
  21. Classification of Human Etiologic Agents on the Basis of Hazard
    a. Oncogenic Viruses, Bacteria, Fungal Agents, Parasitic Agents, Viruses and Prions (Appendix B of the Recombinant DNA guidelines)
  22. Standard Operating Procedure for Handling Animals Dosed with Toxic Chemicals
  23. Diseases Communicable From Animals to Humans
  24. Recommended Procedures for Preventing the Production of Aerosols
    a. General
    b. Centrifuge Operation
    c. Mixing Operations
    d. Vacuum and Aspirating Equipment
    e. Needles and Syringes
    f. Pipettes
    g. Transfer Loops
  25. Recommended Procedures for the Proper Use of Biological Safety Cabinets
  26. Recommended Procedures for Autoclaving Materials

University of the Sciences in Philadelphia • 600 South Forty-third Street • Philadelphia, PA 19104-4495 • phone: 215-596-8800 • email: safety@usp.edu