AGENTS REQUIRED TO BE AUTOCLAVED OR DECONTAMINATED PRIOR TO DISPOSAL

Biosafety Level-3 (BSL-3) Risk Group -3 (RG-3), higher risk BSL-2 agents and waste containing liquid or solid recombinant DNA, including transgenic animals and plants, and virulent pathogens, must be autoclaved or decontaminated prior to being placed into biohazard waste containers.  Biosafety Level 2 and 1 (RG 2 and 1) must also be autoclaved or decontaminated prior to placing in a biohazard waste container if it presents a risk to individuals handling it or if it could harm humans, animals or the environment if it is accidentally released prior to incineration.

Also, risk group 2 and 1 agents must be autoclaved or decontaminated if it is easily transmitted by a few organisms, transmitted through intact skin, or easily transmitted by aerosol. Some departments may require that all infectous waste be autoclaved prior to disposal in the biohazard waste container.

Otherwise, biohazardous waste is to be packaged and sealed directly in the laboratory and collected by EHRS or Department of Transportation (DOT) trained laboratory personnel for disposal/incineration.


University of the Sciences in Philadelphia • 600 South Forty-third Street • Philadelphia, PA 19104-4495 • phone: 215-596-8800 • email: safety@usp.edu