James R. Johnson, PhD
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James R. Johnson
Associate Professor of Biology
Coordinator, BS in Microbiology
Department of Biological Sciences
Contact Info:
Office: STC 273
McNeil Science & Technology Center
Voice: 215.596.8521
Fax: 215.596.8710
Email: j.johnso@usciences.edu
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Training and Degrees
Ph.D.., Biochemistry, Duke University, Durham, NC, 1974
B.S. Chemistry, Auburn University, Auburn, AL
Prior Positions
Research Associate Professor, Univ. of the Sciences in Phila., 1993-Present
Associate Professor of Biology, Univ. of the Sciences in Phila., 1990-Present
Associate Professor of Microbiology, Miami University, 1984-1990
Assistant Professor of Biology, Texas A&M University, 1977-1984
Postdoctoral Research Associate, VA Hospital, Durham, NC, 1974-1977
Membership in Professional Organizations
Phi Eta Sigma Freshman Men’s Honor Society
Phi Lambda Upsilon, National Chemistry Honor Society
Sigma Xi, The Scientific Research Society of North America
American Society for Microbiology
American Society for Biochemistry and Molecular Biology
Research Interests
Molecular mechanisms regulating microbial sulfur metabolism.
Analysis of protein factors and DNA structures which regulate gene activity in Staphylococcus aureus: Escherichia coli K 12.
Selected Recent Publications
Costleigh, J.R. and J. R. Johnson, 2003. Development of a biochemical assay to determine if Staphylococcus aureus is able to reduce the disulfide linkage in cystine. Presented at USciences Fourteenth Annual Sigma Xi Undergraduate Research Symposium, April 2003
Abdullah, I. and J.R. Johnson, 2003. A thin-layer chromatographic method that can be used to detect and measure glutathioine degradation. Presented at the USciences Fourteenth Annual Sigma Xi Undergraduate Research Symposium, April 2003.
Hartzell, S. and J. Johnson, 2002. Comparison of growth of Staphylococcus aureus using varying concentrations of cysteine, cysteine and glutathione and measuring the degradation of the latter compounds using analytical methods. Presented at the USciences Sigma Xi, Thirteenth Annual Undergraduate Research Symposium, April 2002.
Abraham, C. and J. Johnson, 2002. Investigation of cysteic acid, 2-mercaptoethanolamine, and cysteine-sulfinic acid as nutritional substitutes for cysteine or as competitive inhibitors of cysteine metabolism by Staphylococcus aureus. Presented at the USciences Sigma Xi Thirteenth Annual Undergraduate Research Symposium, April 2002.
Collier, C.D. and J.R. Johnson. (1990). the metJ193 allele contains a point mutation which alters the hydrophobic pocket responsible for in vitro binding of S-adenosylmethionine: Effects upon cell growth and induction of met regulon expression. J. Bacteriol. 172:3918-3924.
Bala, G.A., C.D. Collier, M.R. Emmett and J.R. Johnson. (1989). Characterization of two mutant metJ proteins with reduced, temperature dependent capacity to control E. coli K 12 met regulon elements. J. Bacteriol. 171; 4095-4099.